Study Reports & Data Analysis
Getting the most out of your PathHunter study report
PathHunter Primary Study Data Reports
Primary Study results are delivered as two separate data files and include:
1. Study Report - A PDF file containing a project summary, description of the assay protocol, control agonist dose response curves for each target (where applicable), and data matrix (view primary sample report).
2. Data Report – An Excel spreadsheet file containing duplicate raw data values, average % activity or % inhibition of all compound/target interactions and summary tables.
Sample Primary Study Report Data Matrix
PathHunter EC50/IC50 Determination Study Reports (10-point dose response curves)
PathHunter EC50/IC50 Determination Study reports are delivered as two separate data files and include:
1.
Study Report - A PDF file containing a project summary, description of the assay protocol, control agonist dose response curves for each target (where applicable), data matrix which provides EC50/IC50 values for each compound against the tested targets, curve images which display the data points and the best fit curve for each EC50/IC50 determination (
view PathHunter EC50/IC50 sample report).
2.
Data Report - A spreadsheet file containing the raw data values reported from duplicate EC50/IC50 determinations, which can be easily imported into most data management and visualization tools.
Sample Curve Images
Data Analysis & Intrepretation
Definitions
Percent Activity/Inhibition
The results for single concentration (primary screen) for tested compound(s) are reported in your study report and spreadsheets as '% Activity' or '% Inhibition' and are calculated in the following manner:
% Activity(Agonist Mode) =
100% x
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[
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Mean RLU (test sample)- Mean RLU(vehicle control)
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]
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Mean MAX RLU (control ligand)- Mean RLU (vehicle control)
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test sample = client supplied compound
vehicle control = DMSO (0% activity)
control ligand = control compound (100% activity)
% Inhibition (Antagonist mode) = 100% x
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[
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1-
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Mean RLU (test sample)- Mean RLU(vehicle control)
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]
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Mean MAX RLU (control ligand)- Mean RLU (vehicle control)
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test sample = client supplied compound
vehicle control = DMSO (0% activity)
EC80 control = control compound (80% activity)
Dose Response Curve (EC50/IC50)
The results for 10-point dose response curve compound/target interactions are reported in the study report and spreadsheets as EC50/IC50, which are values derived using the Hill equation:
Additional Follow Up Services
Pathunter EC50/IC50 Determination
An ideal follow-up service for Primary Screening. Generate an EC50/IC50 value of compound/target interactions observed in initial PathHunter study data. PathHunter EC50/IC50 determinations are performed as 10-point dose-response curves (in duplicate) and can be requested for any target, making it an ideal tool for primary study follow-up.
- 10-point dose-response curve performed in duplicate
- Consistent & reproducible results
- Can be performed against any of our assays