Technology > Pathways

Technology - Pathway Assays

PathHunter® Pathway Assays are novel, non-imaging functional whole cell assays for measuring multiple cellular signaling events such as protein translocation, secretion, degradation and expression using highly sensitive and quantitative chemiluminescent detection. This approach can be applied to any cell signaling cascade in which a target protein either degrades, translocates or is secreted. These assays serve as pathway indicators.

 

The PathHunter Cell-based Assay Advantage

  • Single functional readout – applicable to different kinase receptors and families
  • Stable cell lines - increased reliability and reproducibility
  • Full length protein – ideal for large molecule therapeutics and small molecule inhibitors
  • High specificity – tagged protein eliminates background from endogenous proteins
  • High quality reproducible data – superior assay windows & robust performance (Z' > 0.6)


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