Technology
PathHunter® and HitHunter® Screening & Profiling Benefits
Whether you're developing small molecule or novel biologic therapeutics, optimizing lead compounds or investigating safety and off-target liabilities, DiscoveRx can support your discovery efforts with accurate, reproducible data that informs decisions at all stages of discovery and development. With over 600 validated functional whole cell assays for screening and profiling, we have full service solutions for a broad range of target classes including
GPCRs,
NHRs, and
Kinases.
Key Benefits
- Largest commercial functional whole cell assay panel available - over 600 assays
- Flexible access to all the major target classes – GPCRs, NHRs, Kinases
- Pre-validated functional whole cell assays provide more information and biological relevance, ensures high quality results
- Reliable, rapid data turnaround
- Accurate, precise & reproducible data
- Convenient, pre-defined assay panels or personalized panel/target selection
- High-throughput screening – 10,000 to 1,000,000 compound handling capabilities (70,000 wells/day screening capacity)
World's Largest Portfolio of Functional Cell-Based Assays
DiscoveRx offers validated cell based assay solutions for every major drug target class which includes over 600 assays for GPCRs, kinases, proteases, nuclear hormone receptors, transcription factors, and secreted proteins.
- 230 GPCR assays - available in 3 assay platforms
- 18 Cell Based Kinase assays – RTK, CTK and Pathway assays
- 18 Nuclear Hormone Receptor assays
Flexible screening solutions
DiscoveRx provides ready access to a portfolio of more than 600 high-quality functional cell based assays, a suite of integrated solutions, user-defined screening and profiling parameters and flexible service models to support your discovery goals and to maximize program success.
- Preselected assay panels - choose any of our defined assay panels or create your own
- Custom assay panels - build a personalized panel selecting only your assays of interest
- Throughput - easily accommodate 1,000 to 1,000,000 compounds
- Flexible services - screening, selectivity profiling and drug re-positioning
- Multiple business models - fee for service, subscription & prepaid screening models available
Rapid data turnaround
Reliable, quick data turnaround is essential for moving your research programs forward, staying on time and within budget. Standard data turnaround is less than 4 weeks from compound receipt, with 3 week turnaround available for regularly scheduled panel profiling or screening programs and subscription services.
Accurate, precise & reproducible data
Accurate, precise and reproducible screening and profiling data are critical components of the discovery and development process and the cornerstone of a successful outsourcing partnership. All PathHunter® and HitHunter® assays undergo thorough validation prior to being released into production and assay performance is continually monitored to ensure data consistency & longitudinal concordance. A stringent validation process ensures the highest quality data possible. Each data point is performed in duplicate. Strict quality control protocols ensure that any assay results not meeting set specifications will be automatically repeated.
Assay Quality - Reproducibility
Assay Quality Data Reproducibility
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| The panels have been validated for exceptional reproducibility to ensure comparable results from test to test. Plots above compare control activity form runs performed over the course of the year. Excellent correlation observed between panel runs. |
Assay Quality – Uniformity
- Z’ values measure assay noise and predictive value
- High Z’ values reproducibly measured across entire assay panel
- High Z’ values ensure low false positive/negative rates
Assay Quality & Data Uniformity
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| Plate uniformity data for a typical PathHunter screen run in antagonist mode. The results show excellent performance for a cell-based assay with an average Z' of 0.7, 47% inhibition for control antagonist IC50, and overall hit rate of 1.5%. |
Assay Quality – Precision
- Assays have been optimized to provide high precision and low variability
- Positive and negative controls included in every assay to monitor performance and quality
Assay Quality Data Precision
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Specialized Panels
DiscoveRx offers fully validated panels of functional GPCR, NHR and kinase profiling assays which can be utilized for selectivity profiling of lead compounds. We offer specialized panels for common therapeutic areas to support drug repositioning efforts as well as liability panels for toxicity assessment. Based on your needs, we can also perform compound profiling against custom panels.